Reporter

Part:BBa_J100333:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2017-09-20)


repClone 2 Red


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal SpeI site found at 21
    Illegal PstI site found at 2317
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal SpeI site found at 21
    Illegal PstI site found at 2317
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal SpeI site found at 21
    Illegal PstI site found at 2317
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal SpeI site found at 21
    Illegal PstI site found at 2317
    Illegal AgeI site found at 2190
    Illegal AgeI site found at 2302
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1606
    Illegal BsaI.rc site found at 728


Design Notes

The TT hairpin loop and placement of the Bsa1 sites were hindering proper replication and protein expression, leading to their need to be moved.


Source

J100324

References